Journal: Viruses

Article Title: A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein

doi: 10.3390/v15010227

Figure Lengend Snippet: Encoding capability of start codon in the predicted ORF in LvHSSP transcript. ( A ) Diagram of the GFP fusion constructs used for transfection. The start codon ATGGTG of the GFP (GFPwt) gene is mutated to ATTGTT (GFPmut). The start codon ATG of the ORF in LvHSSP transcript is mutated to ATT. ( B ) GFP fluorescence signals in different constructs of transfected Sf9 cells.

Article Snippet: Sf9 cells were purchased from the China Center for Type Culture Collection (Wuhan, China), cultured in Sf-900TM II SFM (Gibco, Grand Island, NE, USA) at 27 °C, and sub-cultured every 3–4 days.

Techniques: Construct, Transfection, Fluorescence

Journal: eLife

Article Title: Binding and sequestration of poison frog alkaloids by a plasma globulin

doi: 10.7554/eLife.85096

Figure Lengend Snippet:

Article Snippet: Cell line ( Spodoptera frugiperda ) , Sf9 insect cell culture , Kemp Proteins , , Proprietary insect cell expression technology.

Techniques: Sequencing, Cell Culture, Expressing, Clinical Proteomics, Generated, Recombinant, Plasmid Preparation, Cloning, Purification, Mutagenesis, Labeling, RNA Sequencing, Produced, Software, Mass Spectrometry, Molecular Weight, Microscale Thermophoresis, Immunohistochemistry

Journal: BMC Medicine

Article Title: Zika vaccines and therapeutics: landscape analysis and challenges ahead

doi: 10.1186/s12916-018-1067-x

Figure Lengend Snippet: WHO – Pipeline Zika virus (ZIKV) vaccines (in preclinical development) (last updated January 2017) January 2017)

Article Snippet: Sf9 cells/Baculo , ZIKV envelope dimers (EnvD) , Novavax, Inc. , No , ZIKV E protein , Matrix M adjuvant or aluminum hydroxide.

Techniques: Virus, Vaccines, Adjuvant, Recombinant, Plasmid Preparation, Construct