Journal: Viruses

Article Title: A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein

doi: 10.3390/v15010227

Figure Lengend Snippet: Encoding capability of start codon in the predicted ORF in LvHSSP transcript. ( A ) Diagram of the GFP fusion constructs used for transfection. The start codon ATGGTG of the GFP (GFPwt) gene is mutated to ATTGTT (GFPmut). The start codon ATG of the ORF in LvHSSP transcript is mutated to ATT. ( B ) GFP fluorescence signals in different constructs of transfected Sf9 cells.

Article Snippet: Sf9 cells were purchased from the China Center for Type Culture Collection (Wuhan, China), cultured in Sf-900TM II SFM (Gibco, Grand Island, NE, USA) at 27 °C, and sub-cultured every 3–4 days.

Techniques: Construct, Transfection, Fluorescence

Journal: eLife

Article Title: Binding and sequestration of poison frog alkaloids by a plasma globulin

doi: 10.7554/eLife.85096

Figure Lengend Snippet:

Article Snippet: Cell line ( Spodoptera frugiperda ) , Sf9 insect cell culture , Kemp Proteins , , Proprietary insect cell expression technology.

Techniques: Sequencing, Cell Culture, Expressing, Clinical Proteomics, Generated, Recombinant, Plasmid Preparation, Cloning, Purification, Mutagenesis, Labeling, RNA Sequencing, Produced, Software, Mass Spectrometry, Molecular Weight, Microscale Thermophoresis, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Functional Relationships between L1CAM, LC3, ATG12, and Aβ

doi: 10.3390/ijms251910829

Figure Lengend Snippet: L1 binds to LC3 and ATG12 via its LIR domain, whereas binding to Aβ is not mediated by the LIR domain. ( A ) Substrate-coated recombinant LC3B or Aβ1-42 was incubated with L1Fc alone or with L1Fc together with Aβ1-42 or the LIR peptide. ( B , C ) Substrate-coated recombinant ATG12 was incubated with different concentrations of L1Fc and CHL1Fc ( B ) or L1Fc in the presence and absence of the LIR peptide, the mutated LIR peptide, and Fc as control ( C ). Via ELISA, binding was determined using horseradish peroxidase-conjugated anti-Fc antibody. Mean values ± SD from three independent experiments carried out in triplicates are shown. * p < 0.05 relative to L1Fc ( A , C ) or CHL1Fc ( B ), # p < 0.05 relative to L1Fc+LIR, Mann–Whitney U-test.

Article Snippet: Recombinant human LC3B (UBI-60-0112-500) and recombinant human ATG12 (PRS-95-125; lot 95-125-β9β4) were from BIOZOL (Eching, Germany).

Techniques: Binding Assay, Recombinant, Incubation, Control, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY